A8481
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 | Immunofluorescence Total 3T3 cells extract was separated on SDS-PAGE and incubated with serial concentrations of Monoclonal Anti-GAMMA Actin (Cat. No. A8481). The antibody was developed with Goat Anti-Mouse IgG, Peroxidase conjugate (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. 1 μg/mL antibody 2. 0.5 μg/mL antibody 3. 0.25 μg/mL antibody 4. 0.125 μg/mL antibody |
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A8481
Sigma
Monoclonal Anti-γ-Actin antibody produced in mouse
~2 mg/mL, clone 2-2.1.14.17, purified immunoglobulin, buffered aqueous solution
| Synonym: | Anti-ACTG1 |
Description
| Immunogen | purified bovine brain cytoplasmic γ-actin and the KLH-conjugated amino terminal peptide of human γ-actin (amino acids 2-15).1 |
| Other Notes | To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/actin. |
| Physical form | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
Properties
| antibody form | purified immunoglobulin |
| clone | 2-2.1.14.17 (monoclonal) |
| form | buffered aqueous solution |
| mol wt | antigen mol wt ~43 kDa |
| species reactivity | canine, human, bovine, mouse, hamster, chicken |
| concentration | ~2 mg/mL |
| application(s) | immunoblotting1: 0.25-0.5 μg/mL using total cell extract of 3T3 cells |
| immunohistochemistry: suitable | |
| indirect ELISA1: suitable | |
| isotype | IgG1 |
| Gene Information | human ... ACTG1(71) |
| mouse ... Actg1(11465) | |
| shipped in | dry ice |
| storage temp. | −20°C |
References
| Cited Reference | 1. Hanft, L.M., et al. Proc. Natl. Acad. Sci. U. S. A. 103, 5385-5390, (2006) Abstract |
